A comprehensive survey of the night-flying moth fauna (Insecta, Lepidoptera) of the Doñana National Park will be provided. The work entails operating light and bait traps throughout the seasons in years 2016-2017 in several locations in the Doñana National Park. A fixed trapping location will be operating near the laboratory building.Transient night trapping activities using lights from a portable generator, solar panels, and bait traps will be deployed across the Doñana National Park in selected habitats. About 5 light and 5 bait traps will be operated continuously. All Lepidoptera specimens from the traps will be identified and entered in databases. Specimens necessary for verifying the identifications will be retained for public collections. We expect to identify about 20,000 individuals, representing about 1,000 species. Also netting and photographing of live individuals will be employed. This will produce a complete inventory of moth fauna in the Doñana National Park. Also diurnal butterfly and moth observations will be made on the side. The work is funded by the EU BON project as demonstration of field and data recording protocols.